Guides, introductions, concepts, courses, principles and how-tos . . .


Three courses from LMCF . . .


A simple web based introduction
Introduction
Microscope configurations
Transmitted light
Fluorescence
Fluorescence microscopes
Stereoscopes
Confocals
Spinning disk confocals		 Two photon excitation
TIRF
Preparing your samples
Objectives
Images
Deconvolution
Image analysis
Other points/FAQs
Short theory-only course
One semester course
BIOLOGY 370: Principles and Practice of Microscopy

LMCF guides to . . .

Sample preparation

Live cell imaging

File formats

Metamorph -
Add a scale bar | Region measurements
Image scaling | Review Multidimensional data
Save as QuickTime or AVI movie

Coverslip thickness

Photoshop

Crashes and reboots

How to establish Kohler illumination

How to clean an objective
Manuals for each microscope are available from the specific microscope webpage


Deconvolution
3D Visualization
Image Quantification