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Capabilities
The facility has a range of microscopes that offer a wide array of
capabilities including:
Fluorescence imaging of a huge range of samples
- Different modalities
for different specimens - Widefield, scanning confocal, 2 Photon,
spinning disk confocal, TIRF
- Filters/excitation for virtually all fluorophores (eg dapi, all fluorescent
proteins, Alexa Fluors, CY dyes)
- Laser
lines available: 351 364 405 458 477 488 496 514 543 561
568 594 633 647 nm.
Transmitted light imaging - DIC,
phase contrast, brightfield, darkfield
- Colour IHC imaging
Imaging large range of sample sizes - From
macroscopic samples on the stereoscope . . .
- To samples requiring 100x objectives on a widefield or confocal
Live-cell imaging
- Temperature,
humidity and CO2 regulation on widefield fluorescence, TIRF and
confocal microscopes
- High-speed resonant scanner for rapid less damaging confocal aquisition
- High-sensitivity EM-CCD camera (95% efficiency) on widefield and TIRF
systems
- Time lapse, including multiposition time lapse
FRAP, FLIP, Photoactivation/conversion
- Measure protein dynamics in living cells
FRET
- Sensitized emission and acceptor photobleaching
- Possible on confocal and widefield systems
- Ratiometric CFP/YFP FRET reporter imaging
High-throughput microscopy - Plate
format acquisition capability on an inverted microscope with motorized
stage
Image processing, analysis and quantification
- Measurement of object intensity, size, number etc
- Object tracking
- 3D measurements and rendering
- Deconvolution
If there are other techniques or capabilities not mentioned here that
interest you, please
contact Sam as it may
be possible to add them to the facility if there is sufficient
interest.
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