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Capabilities
The facility has a range of microscopes that offer a wide array of
capabilities including:
Fluorescence imaging of a huge range of samples
- Different modalities
for different specimens - Widefield, scanning confocal, 2 Photon,
spinning disk confocal, TIRF
- Filters/excitation for virtually all fluorophores (eg dapi, all fluorescent
proteins, Alexa Fluors, CY dyes)
- Laser
lines available: 351 364 405 458 477 488 496 514 543 561
568 594 633 647 nm.
Transmitted light imaging - DIC,
phase contrast, brightfield, darkfield
- Colour IHC imaging
Imaging large range of sample sizes - From
macroscopic samples on the stereoscope . . .
- To samples requiring 100x objectives on a widefield or confocal
Live-cell imaging
- Temperature,
humidity and CO2 regulation on widefield fluorescence, TIRF and
confocal microscopes
- High-speed resonant scanner for rapid less damaging confocal aquisition
- High-sensitivity EM-CCD camera (95% efficiency) on widefield and TIRF
systems
- Time lapse, including multiposition time lapse
FRAP, FLIP, Photoactivation/conversion
- Measure protein dynamics in living cells
FRET
- Sensitized emission and acceptor photobleaching
- Possible on confocal and widefield systems
High-throughput microscopy - Plate format acquisition
- High-throughput image
analysis
Image processing, analysis and quantification
- Measurement of object intensity, size, number etc
- Object tracking
- 3D measurements and rendering
- Deconvolution
In development
- FLIM?
- FCS?
At the moment we don't have Laser capture-microdisection.
If this or other techniques not mentioned here interest you,
please
contact Sam as it may
be possible to add them to the facility if there is sufficient
interest.
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