Zeiss Axio Observer widefield fluorescence microscope

Capabilities: Multi-channel fluorescence images - DAPI, CFP, green, YFP, red and far-red Phase contrast and DIC Ideal for thin fixed specimens (oil objectives) Location: Room 4226 French Family Science Center (613-8097) Cost: $10 per hour User manual: html | pdf Request training | Reserve instrument

Detailed specifications

Microscope:

• Zeiss Axio Observer A1 inverted stand
• Zeiss HBO arc lamp and power supply

Filter cubes:

1. DIC
2. DAPI (FS49: G365, FT395, BP445/50)
3. CFP (FS47: BP436/20, FT455, BP480/40)
4. Green (FS38HE: BP470/40, FT495, BP525/50)
5. YFP (FS46: BP500/20, FT515, BP535/30)
6. Red (FS43HE: BP550/25, FT570, BP605/70)
7. (available) Far-red (BP575-625, FT645, BP660-710)

Objectives:

• 5x/0.16 Ph1 Plan Neofluar(44 03 21) px = 1.2623 micron
• 10x/0.3 Ph1 Plan Neofluar(44 03 31) px = 0.6270micron
• 20x/0.5 Ph2 Plan Neofluar(44 03 41) px = 0.3111 micron
• 40x/0.75 Ph2 Plan Neofluar(44 03 51) px = 0.1562micron
• 63x/1.40 DIC Plan Apochromat(44 07 60) px = 0.1001 micron
• 100x/1.4 Ph3 Plan Apochromat (44 07 81) px = 0.0625 micron

Camera: Hamamatsu Orca ER digital camera & controller

Computer:

• Dell precision 490 Dual core Xeon processor, 4 GB RAM, Dual hard-drives, XP professional SP2 32 bit
• MetaMorph 7.5 system ID 3502