Zeiss Axio Observer widefield fluorescence microscope

LMCF axio observer Capabilities:
  • Multi-channel fluorescence imaging - DAPI, CFP, green, YFP, red and far-red
  • Phase contrast and DIC optics
  • Ideal for thin fixed specimens (oil objectives)
  • Multi-well plate holder for TC cell documentation

Location: Room 4226 French Family Science Center

Cost: $11.80 per hour base rate, discounted for bulk use

User manual: html

Request training | Reserve instrument

Detailed specifications

Microscope:
  • Zeiss Axio Observer A1 inverted stand
  • Manual stage with insert for slides, dish and multi-well plates
  • Zeiss HBO arc lamp and power supply
Filter cubes:
  1. DIC
  2. DAPI (FS49: G365, FT395, BP445/50)
  3. CY5 (filter set 50 BP640/30, FT660, BP690/50)
  4. Green (FS38HE: BP470/40, FT495, BP525/50)
  5. YFP (FS46: BP500/20, FT515, BP535/30)
  6. Red (FS43HE: BP550/25, FT570, BP605/70)
  7. (available) CFP (FS47: BP436/20, FT455, BP480/40)
  8. (available) CY7 (BP710/75, FT750, BP810/90)

Manual 6-position fluorescence attenuator - 100, 70, 50, 40, 20 and

Objectives:
  • 5x/0.16 Ph1 440321-9902: EC Plan-Neofluar, NA: 0.16, air, Ph1, WD: 18.5mm
  • 10x/0.3 Ph1 440331-9902: EC Plan-Neofluar, NA: 0.3, air, Ph1, WD: 5.2mm
  • 20x/0.5 Ph2 440341-03: EC Plan-Neofluar, NA: 0.50, Ph2, WD: 2.0mm, coverglass: 0.17mm
  • 40x/0.75 Ph2 440351-9903: EC Plan-Neofluar, NA: 0.75, air, Ph2, WD: 0.71mm, coverglass: 0.17mm
  • 63x/1.40 DIC 420760-9905: Plan-Apochromat, NA: 1.40, oil, DIC, WD: 0.19mm, coverglass: 0.17mm, Thread type: M0.8X1/36"
  • 100x/1.4 Ph3 440781-02: Plan-Apochromat, NA: 1.40, oil, Ph3, WD: 0.17mm, coverglass: 0.17mm
  • 5x/0.16 px = 1.2623 micron
  • 10x/0.3 px = 0.6270micron
  • 20x/0.5 px = 0.3111 micron
  • 40x/0.75 px = 0.1562micron
  • 63x/1.40 px = 0.1001 micron
  • 100x/1.4 px = 0.0625 micron

 

Camera: Hamamatsu Orca ER digital camera & controller

Computer:
  • Dell Optiplex 7010 I7 3770 8GB RAM Win7-32
  • MetaMorph 7.7.3 system ID 3502