Zeiss Axio Imager widefield fluorescence microscope

Duke LMCF Zeiss Axio Imager microscope Capabilities:
  • Widefield fluorescence imaging
  • Motorized stage for stitching/tiling
  • Transmitted light images - brightfield, DIC
  • Colour camera for IHC (e.g. H&E staining)
  • Z-stacks
  • Apotome for optical sectioning

Location: Room C171 LSRC (613-8217)

Cost: $11.80 per hour base rate, discounted for bulk use

User manual: html

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Detailed specifications

Upright AxioImager.Z2 microscope stand

Motorized stage - 75 x 50

Apotome.2 - optical sectioning using structured illumination

HXP 120 Metal halide fluorescence illumination with intensity control. LED transmitted light illumination.

Filter cube sets (10 position turret):

  1. DIC analyzer
  2. DAPI, Hoechst (Chroma 31000v2 D350/50x, 400DCLP, D460-50)
  3. CFP (Chroma 31044v2 D436/20, 455DCLP, D480/40)
  4. Green, GFP, FITC, Alexa488 (Zeiss Filter Set 38 HE 489038-9901-000, BP 470/40, FT 495, BP 525/50)
  5. YFP (Chroma 41028 HQ500/20, Q515LP, HQ535/30)
  6. Rhodamine (Zeiss cube set 43 HE, BP 550/25, FT 570, BP 605/70)
  7. Texas Red (Chroma 41004 Alexa 568, Alexa 594 HQ560/55, Q595LP, HQ645/75)
  8. CY5 Zeiss Filter Set 50 (488050-9901-000), BP 640/30, FT 660, BP 690/50
  9. Brightfield (i.e. empty)
Objectives:
  • 5x/0.16 Plan-Apochromat, NA: 0.16, air, WD: 12.1 mm, M27
  • 10x/0.45 Plan-Apochromat, NA: 0.45, air, WD: 2.0 mm M27
  • 20x/0.80 DIC :Plan-Apochromat, NA: 0.80, air, WD: 0.55 mm, coverglass: 0.17 mm M27
  • 40x/0.9 DIC : EC Plan-NeoFluar, NA: 0.9, air, WD: 0.41 mm, coverglass: 0.17 mm M27
  • 63x/1.4 oil DIC 420760-03: Plan-Apochromat, NA: 1.40, WD: 0.19 mm, coverglass: 0.17 mm, thread type: M27x0.75
  • 100x/1.4 oil DIC 420780-02: Plan-Apochromat, NA: 1.40, WD: 0.17 mm, coverglass: 0.17 mm, thread type: M0.8X1/3

Pixel sizes in microns (both cameras) : 5x 0.91um, 10x 0.45um, 20x 0.23um, 40x 0.11um, 63x 0.072um, and 100x 0.045um.

Cameras: Dual camera ports

  • Axiocam 506 color
  • Axiocam 506 mono

Computer:

  • Zeiss Imaging workstation Win7, 32 GB RAM
  • Zen 2 with modules for z-stacks, Tiling, Autofocus.

This system was substantially upgraded in October 2015. Details of the old system are here for reference.