Zeiss Axio Imager widefield fluorescence microscope

Duke LMCF Zeiss Axio Imager microscope Capabilities:
  • Imaging UV and VIS fluorophores (eg Dapi, CFP, GFP, YFP, Alexa 488/568/633)
  • Transmitted light images - brightfield and DIC
  • Colour imaging for IHC
  • Ideal for thin fixed specimens (oil objectives)
  • Z-stacks

Location: Room C171 LSRC (613-8217)

Cost: $10 per hour

User manual: pdf | html | quick_guide.pdf

Request training | Reserve instrument

Detailed specifications

Upright AxioImager.A1 microscope stand

Mercury arc lamp: Zeiss HBO100 power supply & lamp housing

Filter cube sets (turret):

  1. Multipass dichroic (Semrock Pinkel r381-392/t420-460, r475-495/t510-531, r547-572/t589-623, r643-656/t677-722)
  2. DAPI, Hoechst (Chroma 31000v2 D350/50x, 400DCLP, D460-50)
  3. CFP (Chroma 31044v2 D436/20, 455DCLP, D480/40)
  4. Green, GFP, FITC, Alexa488( Chroma 41017 Endow GFP HQ470/40, Q495LP, HQ525/50)
  5. YFP (Chroma 41028 HQ500/20, Q515LP, HQ535/30)
  6. Red (Chroma 41004 Texas Red, Alexa 568, Alexa 594 HQ560/55, Q595LP, HQ645/75)
Chroma 41001 FITC/Bodipy/Fluo3/DiO (HQ480/40, Q505LP, HQ535/50) available (GFP very similar)

Excitation filters (filterwheel A):

Use with Semrock quadpass dichroic

  1. Open (no filter)
  2. Closed
  3. 485/20 (FITC, Alexa 488)
  4. 560/25 (TRITC, Alexa 568)
  5. 387/11 (DAPI)
  6. 650/13 (Cy5, Alexa 647)
  7. 425/40

Neutral density filters (filterwheel B):

  1. Closed
  2. Open (100% Transmission)
  3. 80% T
  4. 60% T
  5. 40% T
  6. 20% T
  7. 10% T

LUDL emission filterwheel:

  1. Open
  2. 684/24
  3. 624/40
  4. 531/22
  5. 447/60
  6. Analyzer

(Semrock Pinkel multipass emitter was 440/40, 520/21, 606/34, 699/45)

Objectives:
  • 5x/0.15 (Zeiss Plan-NeoFluar) px = 1.2522 micron for Orca
  • 10x/0.30 (Zeiss Plan-NeoFluar) px = 0.6261 micron for Orca
  • 20x/0.50 DIC (Zeiss Plan-NeoFluar) px = 0.3120 micron for Orca
  • 40x/0.75 DIC (Zeiss Plan-NeoFluar) px = 0.1520 micron for Orca
  • 63x/1.4 oil DIC (Zeiss Plan Apochromat) px = 0.0976 micron for Orca
  • 100x/1.4 oil DIC (Zeiss Plan Apochromat) px = 0.0593 micron for Orca

Cameras:

  • Hamamatsu Orca ER monochrome cooled-CCD camera with IEEE (firewire)
  • QImaging MicroPublisher 5.0 MP colour camera

Computer:

  • Dell Optiplex 745 Intel Core 2 Duo processor E6600 2.40GHz, 4 GB RAM, Windows XP Pro 32 bit
  • MetaMorph 7.5, system ID 3551