Zeiss 880 Airyscan inverted confocal microscope

Zeiss 780 fixed stage upright confocal axio examiner Capabilities:

Location: Room 4227 French Family Science Center

Cost: $27 per hour base rate, discounted for bulk use

User manual: coming soon

Request training | Reserve instrument

Detailed specifications:

Microscope:

  • Zeiss Axio Observer Z1 with Definite Focus2
  • Motorized XY stage
  • Metal halide fluorescence source for widefield eyepiece visualization
  • Dapi, Green, Red fluorescence cubes

Lasers:

  • 405 nm Diode laser
  • Argon/2 (458, 477, 488, 514nm)
  • 561 nm Diode laser
  • HeNe 633nm

Objectives:

  • 10x/0.30 (420340-9901): EC Plan-Neofluar, NA: 0.3, air, WD: 5.2mm
  • 20x/0.80 (420650-9901): Plan-Apochromat, NA: 0.80, air, WD: 0.55mm
  • 40x/1.2 (421767-9971): C-Apochromat, NA: 1.2, Water, Corr (coverglass: 0.14-0.19mm), FCS, WD:0.28mm
  • 40x/1.3 (420462-9900): EC Plan-Neofluar, NA: 1.3, oil, WD: 0.21mm
  • 63x/1.4 (420782-9900): Plan-Apochromat, NA: 1.4, Oil, WD: 0.19mm

Detectors:

  • 34 channel confocal detector array for versatilely
  • GaAsP high QE 32 channel spectral array with unmixing capability
  • 2 standard alkali PMTs in positions for blue and far-red detection
  • Photon counting possible with GaAsP array and PMT2 (cooled)
  • AiryScan detector module and AiryScan Fast capability
  • Transmitted light detector

Computer:

  • HP workstation Z640
  • Windows 7 Professional, 64bit
  • CPU: Intel Xeon ES-2623 v3 3.0 GHz; Memory: 32GB
  • Zeiss Zen 2.3
  • Direct network link to FFSC-64 bit analysis workstation