Zeiss 780 confocal upright fixed stage confocal microscope

Zeiss 780 fixed stage upright confocal axio examiner Capabilities:

Location: Room 112 Research Park II (613-8219)

Cost: $26.50 per hour base rate, discounted for bulk use

User manual: html | pdf

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The Zeiss 780 upright confocal was purchased with a shared instrumentation grant for a system for intravital imaging. Users with such experiments receive booking priority on this system in that they may book up to 14 days ahead. All other users with fixed or other live samples are very welcome to use spare time on the system. We will review this policy as the use of the system develops to ensure the system serves its intended use and also more general use in the most efficient manner.

Detailed specifications

Microscope:

  • Zeiss Axio Examiner with fixed stage (mechanical)
  • 4 Objective turret
  • Metal halide fluorescence source for widefield eyepiece visualization
  • Dapi, Green, Red fluorescence cubes

Lasers:

  • 405 nm Diode laser
  • Argon/2 (458, 477, 488, 514nm)
  • 561 nm Diode laser
  • HeNe 633nm
Objectives:
  • 2.5x/0.075 Dry EC Plan NeoFluar 440310-9903 WD 9.5 mm
  • 10x/0.45 Dry Zeiss Plan-Apochromat 1063-139 WD 2.0 mm
  • 20x/0.80 Dry Zeiss Plan-Apochromat 440640-9903 WD 0.55 mm
  • 20x/1.0 Water W Plan-Apochromat 421452-9800 WD 1.8 mm
  • 63x/1.4 Oil plan-Apochromat 44 07 62 (02) WD 0.19 mm (generally in the drawer)

Dichroic(s):

  • Twin gate dichroic system
  • Low angle of incidence for improved reflection suppression
Detectors:
  • 34 channel confocal detector array for versatilely
  • GaAsP high QE 32 channel spectral array with unmixing capability
  • 2 standard alkali PMTs in positions for blue and far-red detection
  • Photon counting possible with GaAsP array and PMT2 (cooled)
  • Transmitted light detector

Computer:

  • Xeon X5260 3.33 Ghz processor, 8 GB RAM, 1TB RAID data array, 30" monitor
  • Windows 7 SP1 64bit
  • Zen 2010

This system was purchased with funding from the NIH/NCRR Shared Instrumentation Grant 1S10-RR027867-01