Zeiss 780 confocal inverted confocal microscope

duke lmcf zeiss 780 confocal Capabilities:
  • High sensitivity point scanning confocal imaging
  • Spectral array GaAsP detectors
  • Inverted microscope
  • Incubation for live cell timelapse - temp and CO2 control
  • Definite focus for timelapse

Location: 0213A Nanaline Duke

Cost: $27 per hour base rate, discounted for bulk use (money goes to LMCF to help pay for the service contract)

User manual: html

Request training | Reserve instrument

This system was purchased by Vann Bennett who has very generously allowed the system to be managed through LMCF so other people are able to use the system. To ensure Bennett lab members have access to the machine they have paid for, they are able to book time up to 14 days ahead; other users can make reservations up to 7 days ahead.

Vann is interested in all aspects of science so if you see him when you are over in his lab using the system make sure you tell him about your experiment and what you are doing.

Detailed specifications

Microscope:

  • Zeiss Axio Observer Z1
  • Motorized XY stage
  • Metal halide fluorescence source for widefield eyepiece visualization
  • Dapi, Green, Red fluorescence cubes

Lasers:

  • 405 nm Diode laser
  • Argon/2 (458, 477, 488, 514nm)
  • 561 nm Diode laser
  • HeNe 633nm
Objectives:
  • 10X/0.3 NA Dry Plan-Neofluar (420340-9901)
  • 20X/0.8 NA Dry Plan-Apochromat (420650-9901)
  • 40X/1.4 NA Oil Plan-Apochromat DIC, (UV) VIS-IR (420762-9900)
  • 63X/1.4 NA Oil Plan-Apochromat DIC (420782-9900)

Dichroic(s):

  • Twin gate dichroic system
  • Low angle of incidence for improved reflection suppression
Detectors:
  • 34 channel confocal detector array for versatilely
  • GaAsP high QE 32 channel spectral array with unmixing capability
  • 2 standard alkali PMTs in positions for blue and far-red detection
  • Photon counting possible with GaAsP array and PMT2 (cooled)
  • Transmitted light detector

Computer:

  • X5650 2.67 GHz w/ 6GB RAM, 230 GB Boot, 2 TB Data
  • Windows 7 Ultimate 64bit
  • Zen 2011