Zeiss 710 inverted confocal microscope

Duke LMCF zeiss 710 inverted confocal Capabilities:
  • Imaging diverse fluorophores in fixed or live samples
  • Temperature, humidity, CO2 control
  • 3D z-stacks, timelapse, stitching, multi-position timelapse

Location: Room 337D Nanaline Duke (681-4076)

Cost: $27 per hour base rate, discounted for bulk use

User manual: html

Request training | Reserve instrument

Detailed specifications

Microscope:

  • Zeiss Axio Observer
  • Metal halide fluorescence source for widefield eyepiece visualization
  • Dapi, Green, Red fluorescence cubes

Lasers:

  • 405 nm Diode laser
  • Argon/2 (458, 477, 488, 514nm)
  • 561 nm Diode laser
  • 594 nm HeNe
  • 633nm HeNe
Objectives:
  • 10x/0.45 Dry Plan-Apochromat
  • 20x/0.8 Dry Plan-Apochromat
  • 40x/1.30 Oil EC Plan Neofluar DIC
  • 63x/1.40 oil Plan-Apochromat DIC
  • 100x/1.4 oil Plan-Apochromat DIC

Dichroic(s):

  • Twin gate dichroic system
  • Low angle of incidence for improved reflection supression
Detectors:
  • 34 channel confocal detector array for versatily
  • Transmitted light detector

Computer:

  • X5260 3.33 GhZ 16 GB RAM Boot drive, 2 TB data drive
  • Windows 7 64bit
  • Zen 2009