Zeiss LSM 510 inverted confocal microscope

Capabilities: Imaging UV and VIS fluorophores (eg Dapi, CFP, GFP, YFP, Alexa 488/568/647) Ideal for fixed specimens (oil objectives) Z-sectioning Motorized stage for tiled images Location: Room 4227 French Family Science Center (613-8097) Cost: $25.75 per hour base rate, discounted for bulk use User manual: pdf | html | quick_guide.pdf Request training | Reserve instrument

Detailed specifications:

Microscope:

• Zeiss Axio Observer
• Motorized stage: Marzhauser scan stage DC 120x100 mm (43 20 28)

Lasers:

• 405 nm Diode laser
• Argon/2 (458, 477, 488, 514nm)
• 561 nm Diode laser
• HeNe 594 nm
• HeNe 633nm

Conventional fluorescence filters (turret):

• Analyzer
• DAPI
• Green
• Red

Objectives:

• 10x/0.30 420340-9901: EC Plan-Neofluar, NA: 0.3, air, WD: 5.2mm,
• 20x/0.80 440640-9903: Plan-Apochromat, NA: 0.80, air, WD: 0.55mm, coverglass: 0.17mm. Thread type, M0.8X1/36"
• 40x/0.95 corr 440654-9902: Plan-Apochromat, Corr, NA: 0.95, air, Correction, , WD: 0.45mm, coverglass: 0.13mm-0.21mm
• 40x/1.3 oil 440452-9903: EC Plan-Neofluar, NA: 1.30, Oil, DIC, WD: 0.20mm, coverglass: 0.17mm, Thread type: M0.8X1/36"
• 63x/1.4 oil 440762-9904: Plan-Apochromat, NA: 1.40, oil, DIC, WD: 0.19mm, Coverglass: 0.17mm, Thread type: M0.8X1/36"
• 100x/1.4 oil 440782-9902: Plan-Apochromat, NA: 1.40, oil, DIC, WD: 0.17mm, coverglass: 0.17mm, Thread type: M0.8X1/36"

Dichroic(s):

• NT 80/20
• HFT 405/488/561/633/KP 725
• HFT 405/488/561
• HFT 405/514/594
• HFT 458/514/561
• HFT 458/561
• HFT 458/594
• HFT 405/488/594

PMTs & Emission Filters:

• Ch 1) LP420, LP475, LP505, LP520, LP575, LP615, LP650
• Ch 2) LP420, LP475, LP505, BP420-480, BP465-510 IR, BP475-525, BP505-550
• Ch 3) LP505, LP530, LP575, BP505-550, BP520-555 IR, BP575-615

Computer:

• Zeiss-built Fujitsu Siemens Intel Xeon CPU, 4 GB RAM, Quad hard-drives, CD/DVD burners
• MS Windows XP Professional SP3
• Zeiss LSM 510 ver4.2