Zeiss LSM 510 inverted confocal microscope

duke lmcf zeiss lsm 510 inverted confocal Capabilities:
  • Imaging UV and VIS fluorophores (eg Dapi, CFP, GFP, YFP, Alexa 488/568/647)
  • Ideal for fixed specimens (oil objectives)
  • Z-sectioning
  • Motorized stage for tiled images

Location: Room 4323 French Family Science Center. A key to the rooms is in 4226 of the FFSC.

Cost: $27 per hour base rate, discounted for bulk use

User manual: html | quick_guide.pdf

Request training | Reserve instrument

Detailed specifications:

Microscope:

  • Zeiss Axio Observer
  • Motorized stage: Marzhauser scan stage DC 120x100 mm (43 20 28)
Lasers:
  • 405 nm Diode laser
  • Argon/2 (458, 477, 488, 514nm)
  • 561 nm Diode laser
  • HeNe 594 nm
  • HeNe 633nm
Conventional fluorescence filters (turret):
  • Analyzer
  • DAPI
  • Green
  • Red
Objectives:
  • 10x/0.30 420340-9901: EC Plan-Neofluar, NA: 0.3, air, WD: 5.2mm,
  • 20x/0.80 440640-9903: Plan-Apochromat, NA: 0.80, air, WD: 0.55mm, coverglass: 0.17mm. Thread type, M0.8X1/36"
  • 40x/0.95 corr 440654-9902: Plan-Apochromat, Corr, NA: 0.95, air, Correction, , WD: 0.45mm, coverglass: 0.13mm-0.21mm
  • 40x/1.3 oil 440452-9903: EC Plan-Neofluar, NA: 1.30, Oil, DIC, WD: 0.20mm, coverglass: 0.17mm, Thread type: M0.8X1/36"
  • 63x/1.4 oil 440762-9904: Plan-Apochromat, NA: 1.40, oil, DIC, WD: 0.19mm, Coverglass: 0.17mm, Thread type: M0.8X1/36"
  • 100x/1.4 oil 440782-9902: Plan-Apochromat, NA: 1.40, oil, DIC, WD: 0.17mm, coverglass: 0.17mm, Thread type: M0.8X1/36"

Dichroic(s):

  • NT 80/20
  • HFT 405/488/561/633/KP 725
  • HFT 405/488/561
  • HFT 405/514/594
  • HFT 458/514/561
  • HFT 458/561
  • HFT 458/594
  • HFT 405/488/594
PMTs & Emission Filters:
  • Ch 1) LP420, LP475, LP505, LP520, LP575, LP615, LP650
  • Ch 2) LP420, LP475, LP505, BP420-480, BP465-510 IR, BP475-525, BP505-550
  • Ch 3) LP505, LP530, LP575, BP505-550, BP520-555 IR, BP575-615
Computer:
  • Zeiss-built Fujitsu Siemens Intel Xeon CPU, 4 GB RAM, Quad hard-drives, CD/DVD burners
  • MS Windows XP Professional SP3
  • Zeiss LSM 510 ver4.2