Zeiss LSM 510 upright confocal microscope (decommissioned 09- 2016)

Duke LMCF Zeiss LSM 510 Confocal microscope Capabilities:
  • Confocal Imaging UV and visible fluorophores (eg Dapi, CFP, GFP, YFP, Alexa 488/543/633)
  • True UV laser 351, 364 nm
  • Ideal for living specimens (water objectives)
  • 3D z-stacks
  • Multiposition timelapse

Location: Room 4323 French Family Science Center (613-8154)

Cost: $27 per hour base rate, discounted for bulk use

User manual: html

Request training | Reserve instrument

Detailed specifications:

Microscope stand:

  • Zeiss Axioplan2
Lasers:
  • Argon/2 (458, 477, 488, 514nm)
  • HeNe1 (543nm)
  • HeNe2 (633nm)
  • Coherent Enterprise UV (351, 364nm)
Conv fluorescence filters (turret):
  • Analyzer
  • FITC/GFP
  • Rhodamine
  • UV Band Pass
  • UV Long Pass
Objectives:
  • 10x/0.30 (Zeiss Plan Neo-Fluar)
  • 25x/0.80 multi imm corr DIC (Zeiss Plan Neo-Fluar)
  • 63x/1.20 W corr (Zeiss C-Apochromat)
  • 63x/1.0 dipping
  • 40x/1.4 Oil plan-Apochromat
Dichroic(s):
  • NT 80/20
  • HFT UV/488/543/633
  • HFT UV/488
  • HFT 458/514
  • HFT 477/543
  • HFT 488/543
  • HFT UV (375)
  • HFT 488
PMTs & Emission Filters:
  • Ch 1) LP385, LP475, LP505, LP530, LP560, LP650
  • Ch 2) LP385, BP385-470, BP435-485 IR, BP475-525, BP505-530, LP505
  • Ch 3) LP505, LP560, BP500/20 IR, BP505-530, BP505-550, BP530-600, BP560-615
Computer:
  • Fujitsu Siemens Celsius Intel Pentium D 2.8 GHz CPU, 4 GB RAM, Dual hard-drives, CD/DVD burners
  • MS Windows XP Professional SP2
  • Zeiss LSM 510 ver4.0