Zeiss LSM 510 upright confocal microscope

Capabilities: Imaging UV and VIS fluorophores (eg Dapi, CFP, GFP, YFP, Alexa 488/543/633) Ideal for living specimens (water objectives) Z-sectioning FRAP/FLIP/Photoactivation, FRET Multiposition timelapse Basic temperature control available Location: Room 4323 French Family Science Center (613-8154) Cost: $20 per hour User manual: pdf | html | quick_guide.pdf Request training | Reserve instrument

Detailed specifications:

Microscope stand:

• Zeiss Axioplan2
• Heated stage inset available

Lasers:

• Argon/2 (458, 477, 488, 514nm)
• HeNe1 (543nm)
• HeNe2 (633nm)
• Coherent Enterprise UV (351, 364nm)

Conv fluorescence filters (turret):

• Analyzer
• FITC/GFP
• Rhodamine
• UV Band Pass
• UV Long Pass

Objectives:

• 10x/0.30 (Zeiss Plan Neo-Fluar)
• 25x/0.80 multi imm corr DIC (Zeiss Plan Neo-Fluar)
• 63x/1.20 W corr (Zeiss C-Apochromat)

Dichroic(s):

• NT 80/20
• HFT UV/488/543/633
• HFT UV/488
• HFT 458/514
• HFT 477/543
• HFT 488/543
• HFT UV (375)
• HFT 488

PMTs & Emission Filters:

• Ch 1) LP385, LP475, LP505, LP530, LP560, LP650
• Ch 2) LP385, BP385-470, BP435-485 IR, BP475-525, BP505-530, LP505
• Ch 3) LP505, LP560, BP500/20 IR, BP505-530, BP505-550, BP530-600, BP560-615

Computer:

• Fujitsu Siemens Celsius Intel Pentium D 2.8 GHz CPU, 4 GB RAM, Dual hard-drives, CD/DVD burners
• MS Windows XP Professional SP2
• Zeiss LSM 510 ver4.0